The Gram-positive, spore-forming, anaerobe C. perfringens is commonly present in the intestine of humans and animals, environment and soil (1). Based on their production of four major exo-toxins including alpha, beta, epsilon, and iota, individual isolates of C.perfringens are categorized into ?ve types (A, B, C, D and E) (2), that A and C strains are pathogenic for humans (3). In addition to the major toxin, more than fifteen different toxins are produced by this bacterium which have been identified as the main virulence factors in its pathogenesis. The role of other enterotoxin such as necrotic enteritis B-like toxin (NetB) and beta-2 toxin, in the pathogenesis of C. perfringens-associated disease (CPAD) in humans and animals have been considered more than others(4, 5). Pathogenic strains of C. perfringens considered as the causative agent for broad range of human and animal diseases including food poisoning, antibiotic-associated diarrhea, gas gangrene, necrotic enteritis, necrotizing colitis, sudden death syndrome, and enterotoxemia stems from these toxin (6, 7).C. perfringens Type A (alpha-toxin-producing type) strain is the predominant toxinotype in the environment and is ubiquitous that causes gas gangrene and food-borne diseases in humans (8). About 1-5% of the strains belonging to this type and carrying cpe gene encoding C. perfringens enterotoxin (CPE) that responsible for several important human gastrointestinal (GI) diseases, including C. perfringens type A food poisoning, 5 to 20% of all cases of sporadic non-food-borne diarrhea (SPOR) and for ?10% of all cases of  antibiotic-associated diarrhea (AAD) (9). C. perfringens Type B produce alpha, beta, and epsilon and type D  produce alpha- and epsilon toxin are known cause of necrotic enteritis and fatal enterotoxemia in animals and occasionally in humans(10, 11). Type E produce alpha- and iota toxin has rarely been isolated in humans, its pathogenicity remains unclear (12)